Fig. 4

The impact of NAT1 on metabolism in CRC cells at mRNA level. A DEGs were identified between the sh-NC (N = 3) and sh-NAT1 (N = 3) groups through RNA-seq analysis in CaCO2 and HCT116 cells. Genes with a FC ≥ 2 and a P-value ≤ 0.05 were designated as DEGs using the R package "limma-voom". B A total of 1756 DEGs were identified in both CaCO2 and HCT116 cells. C Functional analysis of these DEGs revealed metabolic differences between the sh-NC and sh-NAT1 groups. D DEGs was significantly enriched in glycolysis, tricarboxylic acid cycle and oxidative phosphorylation pathways. E Expression levels of DEGs involved in oxidative phosphorylation, the citrate cycle, and glycolysis pathways were assessed using qRT-PCR assay via unpaired t-test. Comparison of sh-NC with sh-NAT1 and OE-NC with OE-NAT1 via unpaired t-test. The experiments were independently repeated at least three times. *P < 0.05