Fig. 4
From: Comparative performance evaluation of bisulfite- and enzyme-based DNA conversion methods
Developmental validation of BC and EC DNA conversion methods in terms of fragmentation index based on the recently developed qPCR QC tool qBiCo [18]. Several parameters were assessed including: A repeatability for intra-experimental variation; B reproducibility and sensitivity for inter-experimental variation; effects of C elution and D incubation time in the conversion methods protocol; E artificial methylation of commercial gDNA standards; robustness in terms of F UV treatment and G sonication prior to conversion; inhibition by H hematin and I proteinase; stability of converted gDNA by J storage time and K freeze-thaw cycles. The genomic DNA input for all conditions (C–K) was 100 ng. Missing values for A are displayed at y = 0 in black