Fig. 9

Expression of IL6 gene in Caco-2 differentiated cells treated with IL-1β and AFA. A Histogram showing the IL6 mRNA levels after co-treatments with IL-1β and AFA10/AFA200; B Histogram showing the IL6 mRNA levels after treatments with IL-1β for 24 h after incubation, the culture medium was replaced with fresh medium with or without AFA10/AFA200. C Histogram showing the IL6 released levels after co-treatments with IL-1β and AFA10/AFA200; D Histogram showing the IL6 released levels after pre-treatments with IL-1β for 24 h and then treatment with or without AFA10/AFA200. Data in the histograms are presented as the mean of triplicate experiments. Statistical analysis performed with one-way analysis of variance (ANOVA) and Tukey’s multiple comparisons test. Differences were considered significant at *p < 0.05, **p < 0.01