Fig. 3

MSRE-PCR revealing methylation status of CG sites in the promoter region of IL6 gene, from Caco-2 cells treated with IL-1β, co-treated with IL-1β + AFA10 and IL-1β + AFA200. Densitometry of the bands is shown by histograms, which represent the mean of three experiments. Statistical analysis performed with one-way analysis of variance (ANOVA) and Tukey’s multiple comparisons test. Differences were considered significant at *p < 0.05, **p < 0.01, ***p < 0.005