Fig. 2

Representative MeSAP-DNA fingerprinting and relative scanning densitometry (A, C, E), indicating genomic DNA methylation of Caco-2 differentiated cells: A (from left) untreated (CTR), 25 ng/mL IL-1β 24 h (IL-1β 24 h), co-treatment 25 ng/mL IL-1β 24 h + 10 ng/μL 24 h (co-treatment IL-1β + AFA10), co-treatment 25 ng/mL IL-1β 24 h + 200 ng/μL 24 h (co-treatment IL-1β + AFA200); C (from left) 25 ng/mL IL-1β 24 h + only DMEM 24 h (IL-1β + DMEM), 25 ng/mL IL-1β 24 h + 10 ng/μL 24 h (IL-1β + AFA10), 25 ng/mL IL-1β 24 h + 200 ng/μL 24 h (IL-1β + AFA200); E (from left) untreated (CTR), AFA10 ng/μL treatment 10 ng/μL 24 h (AFA10), AFA 200 ng/μL 24 h (AFA200), and relative graphic representations of densitometry analysis for co-treatments B or subsequent pre-treatments D or treatment of differentiated Caco-2 cells with AFA10 and AFA200 F. Band pattern variation, in terms of intensification/weakening and appearance/disappearance was evaluated by densitometer scanning of mono-digested DNA (MD) in comparison with double-digested DNA (DD)