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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Vitrification affects the post-implantation development of mouse embryos by inducing DNA damage and epigenetic modifications

Fig. 3

NAC supplement reduced the level of DNA damage induced by vitrification. (A) Representative images of ROS staining in blastocysts treated with 1 μM NAC. Scale bar, 100 μm. (B) The fluorescence intensity of ROS in blastocysts treated with 1 μM NAC (n = 10 for Vit, n = 13 for Vit + NAC). ***p < 0.001. (C) Total cell number of E4.5 blastocysts treated with 1 μM NAC (n = 8 for Vit, n = 12 for Vit + NAC). (D) Representative images of γH2A.X staining in blastocysts treated with 1 μM NAC. Scale bar, 50 μm. (E) The fluorescence intensity of γH2A.X in blastocysts treated with 1 μM NAC (n = 5 for Vit, n = 6 for Vit + NAC). *p < 0.05. Con represents control sample; Vit represents vitrified sample

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Clinical Epigenetics

ISSN: 1868-7083

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