Fig. 1

Vitrification impaired pre- and post-implantation development of mouse embryos. (A) The development of E4.5 blastocysts of control and vitrified group. The red arrows denote low-quality blastocysts (n = 4 for Con, n = 4 for Vit). Scale bar, 200 μm. (B) Cell number of E4.5 blastocysts (for total cell number: n = 21 for Con, n = 28 for Vit; for ICM or TE cell number: n = 14 for Con, n = 14 for Vit). (C) The pregnancy frequency at E18.5. (D) The developmental frequency of the decidua at E7.5 (n = 9 for Con, n = 10 for Vit). (E) The live pup frequency at E18.5 (n = 12 for Con, n = 15 for Vit). (F) Fetal weight at E18.5 (n = 33 for Con, n = 41 for Vit). (G) Placental weights at E18.5 (n = 33 for Con, n = 41 for Vit). (H) Placental efficiency at E18.5 (n = 33 for Con, n = 41 for Vit). *p < 0.05, **p < 0.01. Con represents control sample; Vit represents vitrified sample