Fig. 5

Changes in ‘Decisions’ from DKFZ-MC v11b4 to SNUH-MC-91. This figure illustrates the reclassification of 69 cases from DKFZ-MC v11b4 to SNUH-MC-91. Each case is labeled as “Uncertain → Match,” “No match → Match,” “No match → Likely match (LM),” or “No match → No match.” Each case is presented with MRI (T2-weighted FLAIR) and histopathological (H&E) images. Final diagnoses are provided as subheadings for each image set. Tables show detailed information including main genetic alterations obtained from NGS studies with brain tumor-targeted gene panel (SNUH FiRST brain tumor panel). The final diagnosis of each case is DNET A, B, EPN_PFB C, D & G, H), PedHGG E, F & K, L, and HGAP I, J. In Figure I, Rosenthal fibers (blue arrows) are evident. The last four cases were not matched by all methylation classifiers, including our version, which are PGNT with SLC44A1::PRKCA fusion M, N, pilocytic astrocytoma with KIAA1549::BRAF fusion O, P, GBM, IDH-wt Q, R and pineal anlage tumor S, T. Cases that remained unchanged in the ‘Decisions’ were also included in this plot (DKFZ-MC: Deutsches Krebsforschungszentrum-Methylation Classifier; SNUH-MC: Seoul National University Hospital-Methylation Classifier, DNT: low-grade glioma, dysembryoplastic neuroepithelial tumor, EPN: Ependymoma, PNET: primitive neuroectodermal tumor, HGG: high-grade glioma, G3, G4: grade 3, grade 4, IDH-wt, PLEX, PED B: choroid plexus tumor, pediatric type B, pedHGG_RTK1A: Diffuse pediatric-type high-grade glioma, receptor tyrosine kinase type 1A, HGAP: high-grade astrocytoma with piloid feature, ANA PA: anaplastic pilocytic astrocytoma, LGG, PGNT: papillary glioneuronal tumor, Pil. A: pilocytic astrocytoma, ampl: amplification; mut.: mutation, Underbar size of the HE figures: B: 25 μm, D, F, K, N-T: 50 μm, G: 100 μm, I: 20 μm)