Fig. 1

Schematic representation of the MGMT promoter and the study design. The transcript NM_002412.4 of MGMT gene was used as reference sequence. Gray boxes encompass the 5′-untranslated region (UTR), first exon, and first intron of the MGMT gene; violet boxes encompass differentially methylated regions (DMR1 and DMR2). Red arrows indicate the locations of MS-MLPA probes; gray arrows indicate the locations of sSeq primers. Within the gray boxes, DMR1 and DMR2 sequences show CpGs and MS-MLPA probes (violet arrows)