Identification of 17 novel epigenetic biomarkers associated with anxiety disorders using differential methylation analysis followed by machine learning-based validation

Table 2 Summary of the 17 CpG site locations, associated genes, and CpG annotations

Chromosome	Position	Gene symbol	Genomic location	Direction of methylation difference	CpG Annotation
chr1	45,339,798	MUTYH	Intron; Exon; Far Promoter; 5’UTR; Intron–Exon Boundary	Hypo	CpG Shore
chr1	45,339,798	TOE1	Promoter; Far Promoter	Hypo	CpG Shore
chr7	19,708,981	MIR3146	Far Promoter	Hypo	CpG Island
chr7	19,708,988	MIR3146	Far Promoter	Hypo	CpG Island
chr7	19,708,990	MIR3146	Far Promoter	Hypo	CpG Island
chr10	632,863	DIP2C	Intron	Hypo	CpG Island
chr10	119,892,349	SEC23IP	Promoter	Hypo	CpG Shore
chr10	132,767,837	INPP5A	Intron	Hyper	CpG Shore
chr11	64,304,843	ESRRA	Promoter; Far Promoter	Hypo	CpG Island
chr16	30,610,279	ZNF689	Intron; Exon; Promoter; 5’UTR; Intron–Exon Boundary	Hypo	CpG Island
chr16	30,610,288	ZNF689	Intron; Exon; Promoter; 5’UTR; Intron–Exon Boundary	Hypo	CpG Island
chr17	30,379,602	CPD	Exon; Promoter	Hypo	CpG Island
chr17	30,379,604	CPD	Exon; Promoter	Hypo	CpG Island
chr17	30,379,614	CPD	Exon; Promoter	Hypo	CpG Island
chr17	38,297,134	MRPL45	Exon; Promoter; 5’UTR	Hypo	CpG Island
chr17	38,297,137	MRPL45	Exon; Promoter; 5’UTR	Hypo	CpG Island
chr18	46,104,880	ATP5F1A	Promoter	Hypo	CpG Island
chrX	2,583,990	NA	NA	Hypo	CpG Island

If a gene associated with a biomarker has annotations for different genomic locations based on transcripts, all genomic locations are listed with semicolons. The direction of methylation difference is indicated as “Hyper” for hypermethylated biomarkers and “Hypo” for hypomethylated biomarkers. NA = Not Available

ISSN: 1868-7083