Fig. 4

Decreased TET2 expression led to a reduction in TXNIP levels by downregulating 5hmC levels. A 5hmC coverage depth (per base pair per peak per 10 million mapped reads) of 5hmC peaks (− 2 kb to + 2 kb) in TET2-knockdown ALCL cell lines and controls. B The Venn diagram of genes associated with downregulated enrichment peaks in RNA-seq from TET2 knockdown ALCL cell lines, 5hmC downregulated genes identified through hMeDIP-seq in TET2 knockdown ALCL cell lines, and cell cycle-related gene sets (GO: 0051301, GO: 0007049). C The TXNIP mRNA expression in TET2 knockdown ALCL cell lines and controls was determined by qPCR (n = 3). D The TXNIP protein expression in TET2 knockdown ALCL cell lines and controls was determined by Western blot (n = 3). E Representative 5hmC sites in TXNIP genes represented by integrative genomics viewer. F 5hmC enrichment in TXNIP of TET2 knockdown ALCL cell lines and controls measured by hMeDIP-qPCR (n = 3). G The Analysis of TET2 recruitment at the TXNIP gene in TET2 knockdown ALCL cell lines and controls was determined by CHIP-qPCR (n = 3). H Representative IHC staining of TXNIP with in ALCL samples (n = 46) and reactive lymph nodes (n = 24). I IHC score analysis for TXNIP in ALCL samples (n = 46) and reactive lymph nodes (n = 24). J Correlation of TXNIP expression and TET2 expression in ALCL samples (n = 46). hMeDIP, Hydroxymethylated DNA Immunoprecipitation; CHIP, Chromatin Immunoprecipitation. Data were expressed as the mean ± SDs.  *p < 0.05; **p < 0.01; ***p < 0.001