Fig. 3

Effects of L-Ascorbic acid and Bobcat339 on ALCL cell lines. A The 5hmC levels of ALCL cell lines after L-AA treatment were determined by ELISA, compared with PBS-treated control. B TET2 enzymatic activity of ALCL cell lines after L-AA treatment was determined by ELISA, compared with PBS-treated controls. C The cell cycle distribution of ALCL cell lines after L-AA treatment was determined by flow cytometry, compared with PBS-treated controls. D The cell proliferation ability of ALCL cell lines after L-AA treatment was determined by the CCK-8 assay, compared with PBS-treated controls. E TET2 enzymatic activity of ALCL cell lines after Bobcat339 treatment was determined by ELISA, compared with DMSO-treated controls. F The 5hmC levels of ALCL cell lines after Bobcat339 treatment were determined by ELISA, compared with DMSO-treated controls. G The cell cycle distribution of ALCL cell lines after Bobcat339 treatment was determined by flow cytometry, compared with DMSO-treated controls. H The cell proliferation ability of ALCL cell lines after Bobcat339 treatment was determined by the CCK-8 assay, compared with DMSO-treated controls. L-AA, L-Ascorbic acid; DMSO, Dimethyl sulfoxide. Data were expressed as the mean ± SDs. All experiments were repeated three times independently (n = 3). *p < 0.05;**p < 0.01;***p < 0.001