Fig. 2

TET2 knockdown promotes cell cycle and proliferation. A The 5hmC levels in normal T cells, Su-DHL-1 and DL-40 were determined by ELISA. B The TET2 mRNA expression in normal T cells, Su-DHL-1and DL-40 was determined by qPCR. C The TET2 protein expression in normal T cells, Su-DHL-1 and DL-40 was determined by Western blot. D Validation of TET2 knockdown in ALCL cell lines was determined by qPCR. E Validation of TET2 knockdown in ALCL cell lines was determined by Western blot. F The 5hmC levels in ALCL cell lines with TET2 knockdown or negative control were determined by ELISA. G KEGG pathway analysis of DEGs in ALCL cell lines with TET2 knockdown or negative control. H GSEA analysis of G1/S transition of mitotic cell cycle. I The cell cycle distribution of ALCL cell lines with TET2 knockdown or negative control was determined by flow cytometry. J The cell proliferation ability of ALCL cell lines with TET2 knockdown or negative control was determined by the CCK-8 assay. ELISA, Enzyme linked immunosorbent assay, KEGG, Kyoto Encyclopedia of Genes and Genomes; GSEA, Gene set enrichment analysis; DEGs, Differential Gene Expression, CCK8, Cell Counting Kit-8. All experiments were repeated three times independently (n = 3). Data were expressed as the mean ± SDs. *p < 0.05; **p < 0.01; ***p < 0.001