Fig. 5
From: Hypoxia impairs decitabine-induced expression of HLA-DR in acute myeloid leukaemia cell lines
The effect of hypoxia on HMA-induced HLA expression. A The mean Log2 normalised expression for HLA transcripts in MOLM-13 cells following treatment (Untreated—UT, Decitabine—DAC, Azacitidine—AZA) in either normoxic (21% O2) or hypoxic (1% O2) conditions (n = 3). B The per cent of HLA-DR expressing cells (MOLM-13, MV-4-11, and HL-60 s) following HMA treatment (UT, DAC, AZA) alone in 21% O2 (unshaded) or 1% O2 (shaded). C The per cent of HLA-DR expressing cells follows both HMA treatment and IFN-γ supplementation. Data is presented as mean ± standard error of the mean for replicate experiments (MOLM-13 n = 3; MV-4-11 n = 3; HL-60 n = 5). # indicates significant difference between oxygen tensions within each treatment group. * indicates significant difference of treatment from UT control within each oxygen tension. One-way ANOVA (Tukey’s multiple comparisons); # # or **p < 0.001, # or *p < 0.05