Fig. 2

PDAC cells and distinct PR cells characterized through scRNA-seq analysis. A UMAP visualization depicting clusters within PDAC patient samples, with each color-coded area representing an individual cluster. B UMAP plot illustrating 43,057 cells and highlighting the predominant cell types in PDAC, with each color-coded region denoting a specific cell type. C Proportional distribution of each cell type across individual samples. D Identification of cell populations using top five DEGs. Dot color indicates average expression level, and dot size reflects percentage expression. E Expression patterns of well-established marker genes across the identified PDAC cell types. Each violin represents the gene expression density for a particular marker gene within the corresponding cell type, and violin color indicates the cell type. F UMAP plot showcasing PR cells identified by the SCISSOR algorithm, with red and blue dots representing Surv⁻ (poor survival) and Surv⁺ (favorable survival) cells. G, H Distribution of Surv⁻ cell populations (G) and Surv⁺ cell populations (H) across different cell types. PDAC, pancreatic ductal adenocarcinoma; PR cells, prognostic-related cells; UMAP, Uniform Manifold Approximation and Projection; DEGs, differentially expressed genes