Fig. 4

ZNF93 positively regulates the expression of PRPF3 and is highly expressed in HCC. A The ChIP-seq data of ZNF93, the red boxes show a significant binding peak in the promoter region of PRPF3. B The panel shows the chromatin accessibility at the ZNF93 gene locus in 4 pairs of tumor and adjacent normal tissue samples, with red and blue boxes highlighting regions with differential accessibility. C, D The expression of ZNF93 in the HCC cell lines MHCC-97 h, Huh-7, HepG2, Jhh-7, and the normal liver cell line LO2 were investigated through qRT-PCR(C) and western blotting(D). E Representative immunohistochemical staining of ZNF93 protein in 4 pairs of tumor and adjacent normal tissues (scale bar, 100 μm). F, G The expression of PRPF3 was decreased in HCC cells after knocking down ZNF93. H, I The expression of PRPF3 was increased in HCC cells with ZNF93 overexpression