Fig. 2

Identification and expression analysis of differential genes correlated with chromatin accessibility changes positively. A The heatmap showing the differentially accessible regions (DARs) in chromatin and differentially expressed genes(DEGs) across the four pairs of samples. B The Venn diagram shows that PRPF3 and PLIN2 are the common DARs-associated DEGs across the four sample groups. C The correlation analysis between PRPF3 and its corresponding DARs. The Pearson’s correlation coefficient (R) and the corresponding P value are shown. D The panel showing the chromatin accessibility at the PRPF3 gene locus in 4 pairs of tumor and adjacent normal tissue samples, with red and blue boxes highlighting regions with differential accessibility between them. E PRPF3 expression level between HCC and normal samples in TCGA LIHC database. F, G The expression of PRPF3 in the HCC cell lines, including MHCC-97 h, Huh-7, HepG2, Jhh-7, and the normal liver cell line LO2 were investigated using qRT-PCR(F) and western blotting(G). H The Kaplan–Meier survival curves stratified by PRPF3 expression level. I Representative immunohistochemical staining of PRPF3 protein in 4 pairs of tumor and adjacent normal tissues (scale bar, 100 μm)