Fig. 6

Human fibrotic fibroblasts have decreased TET3 expression. A–B MTS and H/E staining and associated graph representing % of the fibrotic area in non-fibrotic and fibrotic human hearts. C and D Confocal representative images and respective graph shows double staining of TET3 and αSMA in non-fibrotic and fibrotic human hearts. The arrow marks represent the fibroblasts positive or negative for TET3. Summarized quantitative findings are shown as mean ± SEM from 5 non-fibrotic, and 5 fibrotic human hearts. Each dot represents fibrotic area quantified from five independent field from each sample. Statistical significance was calculated using nonparametric two-tailed Student’s t test, and p values correspond to *p ≤ 0. 05. Scale bars represent 10 μm