Fig. 4

TGF-β affects cell proliferation, DNA damage, and TET3 expression in vitro in mCFs. A Representative graph shows the effect of TGF-β on cell number in mCFs from three biological experiments at indicative days. B Representative graph shows relative cell proliferation measured by MTT assay in control and TGF-β-treated mCFs from three biological experiments at indicative days. C Representative confocal images the effect of TGF-β on γH2AX and TET3 expression from three biological experiments in mCFs at indicative days. D Associated graph from Fig. 4e shows γH2AX foci in control and TGF-β-treated mCFs from three biological experiments at indicative days. E Relative TET3 mRNA expression upon TGF-β treatment from three biological experiments in mCFs at indicative days. F Fluorescence measurement using Amplex Red assay showing ROS intensity in TGF-β-treated mCFs from three biological experiments. All the represented experiments are done in triplicates, and statistical significance was calculated using one-way ANOVA. n.s. represents non-significant, and P values correspond to *p ≤ 0.05, **p ≤ 0.01, ***P ≤ 0.01