Fig. 1

Mouse fibrotic fibroblasts proliferate despite increased DNA damage. A–B Confocal representative images and respective graphs show double staining of γH2AX (a marker of DNA double-strand breaks) and αSMA (a marker of fibroblast) in 4 weeks of sham and angiotensin-II-treated mouse hearts. C–D Confocal representative images and respective graphs show double staining of ki67 and α- SMA in 4 weeks of sham and angiotensin-II-treated mouse hearts. E–F Confocal representative images and respective graphs showing double staining of TET3 and αSMA in 4 weeks of sham and angiotensin-II-treated mouse hearts. Scale bars represent 20 μm. In total, each dot in the graph represents the fibrotic area analyzed from 5 independent fields from three independent samples (Sham vs Angiotensin-II). Summarized quantitative findings are shown as mean ± SEM from three shams, and 3 angiotensin-II-treated mouse hearts. Statistical significance was calculated using two-tailed Student’s t test, and P values correspond to *p ≤ 0.05, **p ≤ 0.01, ***P ≤ 0.01