Fig. 2

Stimulation with MPL adjuvant modifies a subset of endotype-associated CpGs. A Dot chart of summary statistics from gene ontology enrichment analysis for the MPL-associated differentially methylated regions highlighting significantly enriched pathways (n = 30). The y-axis shows gene set nomenclature, point size reflects total count of differentially methylated genes, points are ranked by the percentage of genes in the set covered, and colored according to false discovery rate adjusted P-value. B Venn diagram of overlapping regions showing counts of unique and overlapping regions. C Bland–Altman visualization of methylation difference after MPL stimulation for 183 MPL-sensitive CpGs. Y-values represent the difference in log2 fold-change (NIAP – IAP) between PBS and MPL condition. X-axis shows mean methylation value for each CpG. Upper and lower 95% confidence intervals are shown as red lines. D Boxplot of median and quartile range for the four most significantly ranked endotype-associated CpGs modified by MPL treatment. DMRs differentially methylated regions, MPL monophosphoryl lipid A, P.DE p-value for over-representation of the GO or KEGG term, Coverage percentage of genes identified within specified pathway. Hypermeth hypermethylated regions. Hypometh hypomethylated regions.*** = P < 0.001, ** = P < 0.05–0.05, ns = P > 0.05, two-sided t-test