Fig. 4

Genome-wide gene transcriptional difference analysis between the young and aging cochleae and TFs prediction related to ARHL. A Heatmap analysis and hierarchical clustering on transcriptome samples using normalized read counts (TPM values). Each row represents a gene, and the TPM values were Z-scaled by row. The scale bar indicates the Z-scaled TPM values. B The scatter plot of differential expression genes. Gray dots represent genes that are not differentially expressed, blue dots represent genes that are differentially downregulated, and red dots represent genes that are differentially upregulated. C GSEA enrichment analysis of transcriptome with high enrichment scores. D Differential binding TFs prediction using the transcriptomic data. E The footprint of top 5 TFs according to the |logFC| was all significantly decreased from young to aged mice genome-wide