Fig. 1
From: DNA methylation and stroke prognosis: an epigenome-wide association study
Study design diagram. Discovery sample was conformed by two cohorts of patients with stroke, BasicMar-1 (450K Illumina Chip) and BasicMar-2 (EPIC Illumina Chip). Replication phase consisted in two cohorts as well (St. Pau-1, 450 K Illumina Chip; and St. Pau-2, EPIC Illumina Chip). In both cases we filtered cases based on strict stringent criteria defined in a previous study (supplemental methods). We then searched Differentially Methylated Positions (DMPs) in the discovery cohort, and those CpG candidates having a p-value < 10–5 were tested for significance in the replication phase. Both stages were meta-analyzed to search for differentially methylated regions (DMRs) and biological pathways. Gene expression analyses were ran for the replicated DMPs. *In the replication phase, patients could not be rule out according to previous functional status and stroke location, as this cohort did not collect these data