Fig. 2
From: Technical considerations in PCR-based assay design for diagnostic DNA methylation cancer biomarkers
Primer and probe quality assessment of all markers and studies in MSP (A), qMSP assays (probe/SYBR) (B). F: forward primer, R: reverse primer, P: probe, n/a: not applicable, *: Molecular beacon probe, additional bases included in evaluation, Ë : Minor groove binder probe, X: probe sequence could not be mapped back to gene.
: optimal design,
: suboptimal, but acceptable design,
: increased risk of technical problems with the primer or probe,
: not scorable because of molecular beacon probe