Fig. 2

Primer selection procedure. a Workflow used for the development of our DNA methylation assay determining HIV-1 DNA methylation in HIV-1-infected patient samples. b Location of the nine different assays on the HIV-1 genome. Red arrows depict first round PCR primer location, green arrows show second round PCR primer location, red bars indicate the location of the four analyzed CpGIs based on Chavez et al. [12]