Fig. 5

Principal component analysis of DNA methylation profiles of iPSC-derived HPCs with and without co-culture. The DNA methylation profiles of iHPCs (d20; donor 1 and 2) and iPSC-derived HPCs from Nishizawa et al. [14] are closely related to those of iHPCs that were further cultured for 2Â weeks with or without stromal support. Additional stromal support (iHPC on MSCs, iMSCs, and TCP, respectively) did not enhance further commitment toward the hematopoietic lineage