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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Differential open chromatin profile and transcriptomic signature define depot-specific human subcutaneous preadipocytes: primary outcomes

Fig. 5

Validation of Abd-specific gene expression and its correlation with active and repressive histone marks. a IGV genome browser view of RNA-seq and ATAC-seq signals on the indicated loci from abdominal (red) and GF (green) preadipocytes. Overlay tracks are presented for each gene loci. b ChIP-qPCR assay in abdominal and GF preadipocytes with H3K4me3 antibody and H3K27me3 antibody at the promoter region of the selected genes. c RT-qPCR assay for HOXA5 and HOXA3 in abdominal and GF preadipocytes. Wilcoxon test *p < 0.05. White squares represent the IgG negative control

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Clinical Epigenetics

ISSN: 1868-7083

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